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Aug 07, 2023

A candidate transporter allowing symbiotic dinoflagellates to feed their coral hosts

ISME Communications volume 3,

ISME Communications volume 3, Article number: 7 (2023) Cite this article

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The symbiotic partnership between corals and dinoflagellate algae is crucial to coral reefs. Corals provide their algal symbionts with shelter, carbon dioxide and nitrogen. In exchange, the symbiotic algae supply their animal hosts with fixed carbon in the form of glucose. But how glucose is transferred from the algal symbiont to the animal host is unknown. We reasoned that a transporter resident in the dinoflagellate cell membrane would facilitate outward transfer of glucose to the surrounding host animal tissue. We identified a candidate transporter in the cnidarian symbiont dinoflagellate Breviolum minutum that belongs to the ubiquitous family of facilitative sugar uniporters known as SWEETs (sugars will eventually be exported transporters). Previous gene expression analyses had shown that BmSWEET1 is upregulated when the algae are living symbiotically in a cnidarian host by comparison to the free-living state [1, 2]. We used immunofluorescence microscopy to localise BmSWEET1 in the dinoflagellate cell membrane. Substrate preference assays in a yeast surrogate transport system showed that BmSWEET1 transports glucose. Quantitative microscopy showed that symbiotic B. minutum cells have significantly more BmSWEET1 protein than free-living cells of the same strain, consistent with export during symbiosis but not during the free-living, planktonic phase. Thus, BmSWEET1 is in the right place, at the right time, and has the right substrate to be the transporter with which symbiotic dinoflagellate algae feed their animal hosts to power coral reefs.

Symbiosis is a powerful evolutionary strategy that combines skill sets from separate species to meet environmental selection challenges as a consortium. In the case of coral reefs the partnership allows abundant growth in nutrient poor waters that would otherwise support very little biomass [3]. Coral reef symbioses are remarkably successful. Although reefs occupy only 0.1% of the marine environment, they support more than 33% of marine diversity [4].

The symbiotic algae in corals use CO2 and light energy to manufacture sugars by photosynthesis. The sugar is fed to the coral, and this symbiotic ‘currency transfer’ [5] underpins the ability of most corals to deposit calcium carbonate and thus build a reef [3]. In turn, the coral host provides its algal symbionts with shelter, CO2, and precious nitrogen. Transfer of photosynthetically fixed carbon from symbiont to host was demonstrated more than 60 years ago by Muscatine and Hand [6], and symbionts provide as much as 90% of the energy used by a coral [3]. Early work, which used symbionts removed from the host, indicated that glycerol was the principal export [7]. However, recent studies using intact partnerships show that glucose is the principal export and suggest glycerol release by symbionts is either an artefact induced by isolating them from their host [8,9,10], or perhaps transported into the symbiosome vacuole as an osmolyte [11]. Thus, we know what form of photosynthate is transferred, and how much transfer occurs, but we know essentially nothing about how transfer occurs.

Transferred photosynthate must cross at least two membranes: the algal cell membrane; and the so-called symbiosome, a vacuole-like membrane created during the phagocytotic uptake of a symbiont by a host [12]. Given that glucose is membrane impermeable, there are probably transporters in one or both membranes to move glucose outwards. We hypothesise that at least one transporter will be in the symbiont cell membrane and will be predominantly active in hospite. Towards identifying the transporter(s), we previously compared gene expression in free-living and symbiotic cells of Breviolum minutum [1], a dinoflagellate symbiont of corals and anemones [13]. Several transporters showed heightened expression in hospite [1], and one—which we call BmSWEET1—is further characterised here.

Exaiptasia diaphana sea anemones of genotype AIMS3 [13] were kept in a walk-in constant temperature room under the constant temperature of 26 °C, 12:12 h light:dark photoperiod cycle, and 15 μmol photons m−2 s−1 (the GBR anemones are light sensitive and prefer low light conditions [13]), along with constant air supply. Anemones were maintained in three replicate 2.4 L plastic containers with sea water (Red Sea Salt, salinity of 34 ppt), under the same conditions. Anemones were fed ad libitum twice a week with freshly hatched Artemia sp. nauplii (hatched overnight under constant air supply) and filamentous algae were removed prior to weekly full water changes.

B. minutum cells were isolated and cultured from a single genotyped E. diaphana anemone [14]. Three replicate subcultures were generated in cell culture flasks (0.2 μm membrane vented cap) with 0.2 μm filtered seawater (FSW) supplemented with 1 x Diago's IMK medium (Novachem). Cultures were kept in a growth chamber (740FHC LED, HiPoint) under a constant temperature of 26 °C, 12:12 h light:dark photoperiod cycle, and 60 μmol m−2 s−1 photons. Cultures were routinely monitored by light microscopy to assess their health (cells are intact, vital, and motile cells are present). The replicate subcultures were kept and maintained for at least six months prior to protein extractions and sampling for immunofluorescence.

The amino acid sequence of BmSWEET1 was deduced from the B. minutum transcriptome (GICE01031994) [1]. Molecular mass was calculated using the ExPASy Compute PI/MW tool (https://web.expasy.org/compute_pi/). Putative transmembrane domains were predicted using the Phobius [15] and HMMTOP [16] programs. A predicted structure of BmSWEET1 was generated using ColabFold [17] and overlayed with the rice OsSWEET2b solved structure [18] using ChimeraX [19].

Antiserum against BmSWEET1 was produced using synthetic antigenic peptides (Mimotopes) and the services of Walter and Eliza Hall (WEHI) Antibody Facility (Bundoora, VIC). Anti-BmSWEET1 was raised against the peptide RKKPDENSPVSPS conjugated with keyhole limpet haemocyanin and used to immunise two different rabbits in an 82-day immunisation and bleed schedule, which included a total of four boosts. IgGs from the rabbit sera were purified using an affinity column (WEHI) and immunoreactivity (efficiency and specificity) against the antigen was assessed by ELISA (WEHI) and western blotting (see below).

Protein was extracted from B. minutum cultures as described [20] with the following modifications. Cells from 20 ml of culture were pelleted by centrifugation at 15,000 rpm for 1 min, then washed with FSW. A second centrifugation was followed by resuspension with FSW with 2% Triton X100 (Sigma). This step was repeated twice. The pellet was further resuspended with extraction buffer (100 mM Tris, 10 mM EDTA, 100 mM NaCl, pH 7.4) supplemented with protease inhibitor (Roche complete EDTA-free PI cocktail). The cells were homogenised using 710–1180 mm acid-washed glass beads (Sigma) and a TissueLyser II (Qiagen) for 90 s at 30 Hz. The beads were removed, and the homogenate was centrifugated at 15,000 rpm for 5 min at 4 °C. Supernatant was kept, and protein concentration determined using Qubit protein assay kit, according to manufacturer instructions (ThermoFisher).

A protein sample of 20–30 μg was boiled (70 °C, 10 min) with Bolt LDS sample buffer (ThermoFisher) and Bolt Sample Reducing Agent (ThermoFisher) in total volume of 40–50 μl. Proteins were separated on Bolt 4–12% Bis-Tris Plus Gel (ThermoFisher) and transferred to a nitrocellulose membrane according to manufacturer's instructions. Membrane was blocked overnight with 5% skim milk in TBS buffer (137 mM NaCl, 2.7 mM KCl, 24.8 mM Tris base, pH 7.4) containing 0.05% Tween20 (TTBS). The membrane was blotted with the primary antibody for 1 h, then washed three times with TTBS for 5 min, and then incubated with Amersham ECL anti-rabbit IgG conjugated to horse radish peroxidase (Bio-Strategy). Three additional washes of 5 min were done before exposing the membrane to SuperSignal West Pico PLUS reagents for 5 min (ThermoScientific).

To verify antiserum specificity, serum was pre-incubated with the peptide antigen (1:1 mole ratio) for 1 h with shaking at room temperature prior to western blotting as above.

Three replicates of cultured free-living and symbiotic (freshly isolated from anemones [1]) B. minutum cells were labelled for immunofluorescence either with anti-BmSWEET1, pre-immune serum, or no primary antibody followed by washes and a secondary, fluorescent labelled antibody as below.

Two ml (~1 × 106 cells) from each of the three replicate flasks were sampled for quantitative immunofluorescence [16]. Cells were pelleted by spinning at 15,000 rpm, 1 min, and washed with 2 ml of FSW. Cells were fixed by incubating with 4% paraformaldehyde (Electron Microscopy Sciences) in phosphate buffered saline buffer (PBS) (137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4), for 3 h at 4 °C. For permeabilization, the cells were incubated with 0.1% Triton X100 in PBS for 10 min. Cells were pelleted and washed three times with of 0.1% Tween20 (Bio-Rad) in PBS (PBST). Then blocking for unspecific binding was achieved by incubating with 1% bovine serum albumin (BSA) (Sigma) in PBST for 2 h. Further, pellet of cells was suspended with the primary antibody diluted in 0.1% BSA in PBST and incubated over-night at 4 °C. Following three washes with PBST, the cells were suspended with goat anti-rabbit IgG, Alexa Fluor 546 (ThermoScientific) 1:500 in 0.1% BSA in PBST, for 2 h, in the dark. Finally, the cells were incubated with DAPI (ThermoScientific) 1:100 in 0.1% BSA in PBST, for 10 min, in the dark.

Symbiont cells were isolated from 20–30 medium-sized anemones of genotype AIMS3, by homogenisation with glass homogeniser and multiple (~10) spins at 15,000 rpm, 1 min, and washes with FSW. This procedure minimised host cells in the resulting homogenate. The rest of the IFA protocol was the same as described above for the cultured symbionts.

Five µl of sample was loaded onto PTFE printed slides (SPI supplies) with ProLong™ Glass Antifade Mountant (ThermoScientific) and visualised on an inverted confocal Nikon A1R microscope. Images were acquired using DAPI and TRITC filters.

Quantification of the intensity of the signal was achieved by further analysing the images via Fiji software (ImageJ) [21], which allowed us to compare BmSWEET1 signal as a proxy for protein amount. Randomly selected cells (at least 100 cells) from each of the three replicates and each treatment were identified based on their shape, size, and chlorophyll autofluorescence. Then, the mean intensity of the stain around the cell was determined based on the fluorescence from the secondary antibody. No significant difference was observed in cell wall thickness for free-living versus symbiotic algal cells (Fig. S1), so we assume that access to the labelling reagents is equivalent.

Statistical analysis for quantitative microscopy was performed using SPSS software (Version 20.0., IBM Corp). Data was tested for normality and equal variances. To distinguish statistically significant results, we used the two-way ANOVA analysis followed by post hoc (Tukey HSD) multiple-comparisons test (p < 0.05).

Three negative controls for immunofluorescence were performed. The first involved labelling with a pre-immune serum, which gave no signal (not shown). The second was preincubation of the serum with the peptide antigen (1:1 mole ratio) for 1 h with shaking at room temperature prior to adding the serum to slides, which resulted in no yellow BmSWEET1 signal (not shown). The third negative control was to omit the primary antibody.

The coding sequences of AtSWEET1 (AT1G21460) and AtSWEET11 (AT3G48740) were amplified from Arabidopsis thaliana cDNA using primers SF46/SF47 and SF52/SF53 (Table S1), respectively. The cDNA sequence of BmSWEET1 was codon optimised for Saccharomyces cerevisiae and synthesised by Integrated DNA Technologies. Synthesised gene fragments were used for PCR with primers SF50/SF51. The PCR fragments were subcloned into pJet1.2 (Thermo Fisher Scientific) and verified by sequencing. After digestion with PacI and XhoI, the fragments were ligated into the yeast expression vector pDR195 [22].

The yeast EBY4000 strain [hxt1–17D::loxP gal2D::loxP stl1D::loxP agt1D::loxP ydl247wD::loxP yjr160cD::loxP], a mutant in multiple hexose importers [23], was transformed with the different pDR195 vectors–containing either AtSWEET1, AtSWEET11, BmSWEET1 as insert, or the pDR195 vector without insert respectively as described [24]. Transformants were selected on YNB medium + \({{{\mathrm{NH}}}}_{4^{+}}\), +His, + Trp, +2% Mal, - Ura, and verified by PCR.

To test for glucose uptake activity, the different yeast strains were precultured overnight in YNB medium (+ His, + Trp, +2% Mal) at 30 °C, 160 rpm. For EBY4000, Ura was added to the medium. Cells were harvested by centrifugation (2500 × g, 10 min, room temperature) and washed twice with TE buffer (10 mM Tris-HCl, pH 8.0, 1 mM EDTA). Optical density was adjusted to OD600 = 3. The yeast strains were spotted as a serial dilution onto YNB medium plates with or without Ura and supplemented with either 2% Mal or Glc as a carbon source. Additionally, the effect of 0.2% 2-deoxy-D-glucose was tested. Growth was documented by photography after 3 d incubation at 30 °C or 5 d incubation at 30 °C with Fru as a carbon source.

BmSWEET1 (GICE01031994) has high sequence similarity to members of a transporter family known as SWEETs (sugars will eventually be exported transporters) (Fig. 1A). SWEETs were first discovered in plants [25], where their roles include exporting sugars from leaves for transport down to the non-photosynthetic roots, loading sugars into seeds to give new plants a start in life, and secretion of sugars into flower nectaries to attract pollinators [25, 26]. Since their initial discovery in plants, SWEETs have subsequently been identified in animals [27], fungi [28], protists [28, 29], and bacteria [28, 29]. Eukaryotic SWEETs apparently arose through fusion of two prokaryotic half transporters known as semi-SWEETs [28]. The structure of eukaryotic SWEETs, with two triple-helix bundles connected via an inversion linker helix (i.e., seven transmembrane domains) [18, 28], is consistent with fusion of two semi-SWEET genes [18, 28]. SWEETs are bidirectional uniporters that facilitate diffusion of sugars down a concentration gradient [25, 30]. In plants the substrates can be glucose, fructose, and sucrose [25, 30].

A Alignment of BmSWEET1 with OsSWEET2b (rice SWEET2b) showing high levels of sequence similarity (* [asterisk] indicates positions with fully conserved residue,: [colon] indicates conservation between groups of strongly similar properties, and. [period] indicates conservation between groups of weakly similar properties [19]). Two MtN3 domains in BmSWEET1 are indicated by pink bars. Predicted transmembrane helices in BmSWEET1 are indicated by grey text. Tyrosine 61 (green) at the extrafacial gate of OsSWEET2b (17) is conserved in BmSWEET1, as are three of the four prolines (red) lining the OsSWEET2b substrate tunnel (17). The asparagine pair (lower case n) at the substrate selection site in OsSWEET2b (17) is not evident in BmSWEET1 from this alignment. The peptide used to produce anti-BmSWEET1 serum is indicated by a red line. B Solved structure of rice OsSWEET2b showing seven transmembrane helices [18]. C ColabFold predicted structure of BmSWEET1 showing eight transmembrane domains. The approximate position of the peptide used to generate the BmSWEET1 antiserum is shown as a red line. D Overlay of the OsSWEET2b (beige) and BmSWEET1 (blue) structures showing extensive co-linearity plus an extra, N-terminal transmembrane domain (right hand side) in BmSWEET1.

BmSWEET1 has primary structure hallmarks of a typical eukaryotic SWEET, namely two MtN3/saliva domains and seven predicted transmembrane helices [18, 25]; a possible eighth transmembrane domain is predicted in an N-terminal extension of BmSWEET1 not shared with plant SWEETs (Fig. 1A). Alignment [31] of BmSWEET1 with the rice SWEET2b (OzSWEET2b) shows a high level of sequence identity (Fig. 1A), and conserved features such as the tyrosine at the external gate [18, 25], and three of four prolines that line the transport pathway [18, 25], are evident in BmSWEET1 (Fig. 1A). However, the pair of asparagine residues surrounding the glucose substrate recognition site in OsSWEET2b [18] is not evident in BmSWEET1 (Fig. 1A). We used Alpha Fold [17] to predict a structure of BmSWEET1 (Fig. 1C), and we compared the predicted dinoflagellate protein structure with the solved structure for rice, OsSWEET2b [18] (Fig. 1B). AlphaFold corroborated the existence of eight transmembrane domains in BmSWEET1 (Fig. 1C). The predicted BmSWEET1 structure is very similar the OsSWEET2b structure [18] with seven transmembrane helices overlapping almost exactly (Fig. 1D). The additional (eighth) predicted transmembrane in BmSWEET1 protein lies to the right hand side in this view (Fig. 1C) and would result in the N-terminus being internal, which contrasts to the rice protein in which the N-terminus is extracellular [18]. What role the extra (eighth) transmembrane plays in BmSWEET1, and whether BmSWEET1 exists as a homotrimer like the rice glucose facilitative transporter [18], remain to be determined. Nevertheless, the similarity of the two proteins (Fig. 1D) is remarkable considering that dinoflagellates and plants last shared a common ancestor ~2b years ago [32]. Based on sequence analysis and structure modelling, we consider it extremely likely that BmSWEET1 is a bidirectional sugar uniporter with indeterminate substrate preference(s).

Five additional SWEET-like genes are also evident in the B. minutum genomic resources [2] (Table S1). None of them were upregulated in hospite [1], and they are not characterised further here. Importantly, we also found SWEET-like genes in other symbiotic dinoflagellates for which genomic resources are available (Table S1).

BmSWEET1 is predicted to be a cell membrane protein by WoLF PSORT [33], and our next step was to localise the protein. Antisera to a peptide (RKKPDENSPVS) unique to BmSWEET1 (Fig. 1A, C) decorates a single band with an apparent mass of 54 kDa in western blots of SDS-PAGE separated B. minutum proteins (Fig. 2A). Preincubation of the serum with the peptide abrogated binding of the serum to this band (Fig. 2B), demonstrating that the serum is specific for the BmSWEET1 peptide. The apparent mass of BmSWEET1 is in reasonable agreement with the predicted mass (35 kDa) considering that membrane proteins often migrate aberrantly in SDS-PAGE [34].

A The peptide antiserum labels a single band of molecular mass 56 kDa in cultured cells. B labelling is abrogated by preincubation of the serum with the antigenic peptide. C Ten cultured B. minutum cells labelled with DAPI in blue. D BmSWEET1 antiserum in yellow. E Chlorophyll autofluorescence in red. F Bright field illumination. G Merge of all, showing BmSWEET1 at the periphery of the cells and not associated with the nuclei or the plastids. H Close up of a single cultured B. minutum cell stained with calcofluor for cellulose in magenta. I Same cell as H labelled for BmSWEET1 in yellow, J Same cell as H showing chlorophyll autofluorescence in red. K Same cell as H in bright field illumination. L Merge of calcofluor and BmSWEET1 showing the BmSWEET1 protein mostly subtending the cellulose wall. M Electron micrograph of a B. minutum cell depicting the cell membrane situated just underneath the cell wall.

Immunofluorescence assays using this serum show that BmSWEET1 is located at the periphery of the dinoflagellate cells, and not associated with the blue, DAPI-stained nuclei (Fig. 2C). BmSWEET1 is distal to the red chlorophyll autofluorescence defining the plastids and likely not a chloroplast membrane protein (Fig. 2E–J). Co-staining with calcofluor white, which we used to stain cellulose magenta, reveals that the BmSWEET1 protein is situated at or just beneath the outer cell wall (Fig. 2H–M), most likely in the dinoflagellate cell membrane. We conclude that BmSWEET1 is predominantly a cell membrane (plasma membrane) protein of B. minutum.

To determine whether BmSWEET1 is indeed a sugar transporter, and what its preferred substrate is, we applied the widely used yeast mutant complementation assay [18, 25, 26, 28]. The yeast hexose transporter mutant EBY4000 cannot import glucose and fails to grow on glucose agar [23]. EBY4000 can be transformed with expression plasmid pDR195, which will confer the ability to grow on glucose media if it expresses a glucose importer. Because SWEETs are bidirectional facilitators able to transport sugars from high to low concentrations, yeast expressing a functional SWEET can take up select sugars from the medium [18].

Transformants expressing BmSWEET1, Arabidopsis thaliana SWEET1 (a glucose transporter [25]), AtSWEET11 (an Arabidopsis sucrose transporter [26]), and empty pDR195 vector (containing no transporter gene) were selected on solid SD medium with 2% maltose as the carbon source at 30 °C for 4 days, then spotted as a dilution series on either maltose or glucose plates, both of which were minus uracil to select for pDR195 containing strains (pDR195 also expresses orotidine-5′-phosphate decarboxylase [URA3], which is required for uracil biosynthesis).

All strains grew on maltose plates supplemented with uracil (Fig. 3A). Untransformed EBY4000 did not grow on maltose plates that lack uracil (Fig. 3B). As expected, the empty vector yeasts (pDR195) were able to grow on the maltose plates (Fig. 3A, B), but not glucose plates (Fig. 3C). Similarly, the AtSWEET11 expressing yeasts grew on the maltose plates (Fig. 3A, B) but not glucose plates (Fig. 3C), which is consistent with AtSWEET11 being a sucrose transporter [26]. Conversely, yeast expressing the known glucose transporter AtSWEET1 [25] grew on glucose (Fig. 3C). Yeast expressing BmSWEET1 also grew on glucose (Fig. 3C), confirming that BmSWEET1 is a glucose transporter.

A Untransformed yeast strain EBY4000 and strains transformed with the expression plasmid pDR195 harbouring the Arabidopsis glucose transporter AtSWEET1, the Arabidopsis sucrose transporter AtSWEET11, or the B. minutum putative transporter BmSWEET1, or empty vector all grow on maltose (Mal) plates supplemented with uracil (Ura). B If uracil is omitted from maltose plates, the untransformed mutants (EBY4000) fail to grow as they are auxotrophic for uracil. C If maltose is replaced by glucose (Glc) only yeast expressing a glucose importer can survive, namely AtSWEET1 transformants and BmSWEET1 transformants. D Conversely, if maltose substrate is supplemented with a toxic glucose analogue (2-dexoy-D-glucose), any hexose import-competent strains (i.e. AtSWEET1 transformants and BmSWEET1 transformants) will die, while hexose import incompetent strains (AtSWEET11 and pDR195 empty vector) can grow using maltose and are not poisoned by 2-dexoy-D-glucose. E When fructose (Fru) is supplied as the carbon source, AtSWEET1 transformants (which can import fructose) grow, as do BmSWEET1 transformants to a lesser degree. Images of the entire dilution series plates are shown in Fig S1.

As a further test of glucose transport by BmSWEET1, we employed the toxic glucose analogue 2-deoxy-D-glucose (Fig. 3D). Yeast transformants plated on maltose plus 2-deoxy-D-glucose die if they are glucose import competent. Thus, yeasts expressing AtSWEET1 or BmSWEET1 did not grow in this assay whereas those expressing AtSWEET11, or no transporter (empty vector) grew successfully because they could not take up the toxic glucose analogue (Fig. 3D).

We also tested the ability of BmSWEET1 to transport fructose (Fig. 3E). Yeasts expressing AtSWEET1 grew on fructose as expected [35], and limited growth by yeasts expressing BmSWEET1 was also evident on fructose (Fig. 3E), indicating some capacity of BmSWEET1 to transport fructose. We conclude that the optimal substrate of the BmSWEET1 transporter is glucose.

We next sought to corroborate transcriptome evidence that BmSWEET1 is more abundant when the algae are symbiotic within an animal host [1, 2] by using our antiserum to measure protein levels. Like other SWEETs, BmSWEET1 is apparently an unregulated facilitator of passive diffusion, so expressing a glucose exporter in the free-living, planktonic state would appear maladaptive. Free-living cells are unlikely to export precious glucose to the ocean, and leaking glucose to the environment could also attract algivorous predators [36]. Conversely, symbiotic cells would be expected to express more BmSWEET1 to fulfil their part of the symbiotic ‘currency exchange’ [5].

We attempted to measure the amount of BmSWEET1 in both free-living and symbiotic algae using western blotting, but the lack of antibodies to other constitutively expressed dinoflagellate proteins to use as a loading control, and high background levels in blots of isolated symbionts (apparently caused by anemone contamination) obviated this approach. We therefore decided to quantify immunofluorescence labelling of BmSWEET1 in individual cells—either free-living B. minutum cells, or symbiotic cells of the same strain freshly isolated from the anemone host E. diaphana (Fig. 4).

A-C Breviolum minutum cells cultured in vitro. D–F Cells of the same strain freshly isolated from host anemones. Both sets of cells were immunostained for BmSWEET1 protein and imaged in parallel to compare labelling intensities. A BmSWEET1 labelling in free-living cells showing relatively weak BmSWEET1 labelling is depicted in yellow. B Bright field illumination. C Merge of all. D B. minutum cell symbiotic in a host anemone showing intense BmSWEET1 labelling. E Bright field illumination. F Merge of all. G–J Immunostaining of the two populations (free-living versus in hospite) was performed three times, and labelling intensity was measured for >100 cells (n) and depicted as box/whisker plots. G Fluorescence intensity in the free-living cells. H Fluorescence intensity of the in hospite cells. I, J Background fluorescence intensity was quantified in equivalent numbers of cells prepared similarly but without the primary antiserum and is marginally less than was measured for free-living cells labelled with the primary antibody (compare G).

The antibody labelling and imaging of the two samples of algae were done in parallel with the same conditions and microscope settings (Fig. 4A–F). Labelling and quantitative comparisons were done three separate times, and fluorescent intensity of >100 cells were quantified for each treatment (Fig. 4G–J). Baseline/background labelling was established by omitting the primary BmSWEET1 antiserum (Fig. 4I, J). Quantitation shows that the algae have significantly more BmSWEET1 protein in hospite (Fig. 4H) than they do whilst free-living (Fig. 4G). To control for differential accessibility of antibodies to the free-living cells versus the freshly isolated symbiotic cells, we measured cell wall thicknesses, which showed that the median wall thickness did not differ in the two groups (Fig. S2). The symbiotic dinoflagellates exist at lower light intensity (15 μmol m−2 s−1 photons) than their free living, in vitro cultured counterparts (60 μmol m−2 s−1 photons, see Materials & Methods). To control that light intensity was not affecting the amount of BmSWEET1 protein, we performed immunofluorescence on two cultures: one grown at 60 μmol m−2 s−1 photons, and another at 15 μmol m−2 s−1 photons. No significant difference in BmSWEET1 immunofluorescence labelling was observed at different light intensities in vitro (Fig. S3).

Interestingly, the labelling levels in the free-living state (Fig. 4G) are barely more than the negative controls in which the primary antiserum was omitted (Fig. 4I, J), which is consistent with our hypothesis of minimal BmSWEET1 expression during this life phase to minimise wasteful glucose export or danger of attracting algivores [36]. Host secreted factors are believed to induce export of fixed carbon from dinoflagellate symbionts [37,38,39], and various plant pathogens are able to upregulate plant SWEETs to gain access to host sugar [40], so it will be interesting to determine what causes the heightened expression of BmSWEET1 in hospite.

We conclude that BmSWEET1 is upregulated, and perhaps more stable, in symbiotic algae, which is consistent with it being an exporter of glucose able to feed its host.

We focused on a putative glucose transporter, BmSWEET1, in the symbiotic dinoflagellate B. minutum that had previously been shown to be upregulated at the gene expression level in hospite compared to free-living [1]. Sequence analysis and structure modelling suggested BmSWEET1 is a facilitative sugar transporter that would assist sugar to diffuse down a concentration gradient. We localised BmSWEET1 to the symbiont cell membrane. We showed that BmSWEET1 transports glucose by complementing a hexose import deficient yeast mutant. Finally, we showed by quantitative microscopy that B. minutum dinoflagellates living within anemone hosts harbour substantially more BmSWEET1 in their cell membranes than free-living cells of the same strain.

BmSWEET1 thus has three properties making it a likely candidate for the symbiotic exporter of glucose in dinoflagellate-invertebrate symbioses: i/ appropriate location (algal cell membrane), ii/ right substrate (glucose), and iii/ markedly higher expression level in hospite. With dinoflagellate photosynthesis as a glucose source, and host animal respiration as a glucose sink, BmSWEET1 is an ideal gateway to facilitate the diffusion of glucose from endosymbiont towards its host and thus feed corals to underpin the creation of coral reefs.

Materials described in the manuscript, including all relevant raw data, are freely available upon request.

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This study was supported by Australian Research Council Discovery Grants (DP160101539 to GIM and MvO, DP210100639 to GIM, and DP180102630 to MJHvO), and Australian Research Council Laureate Fellowships (FL170100008 to GIM, and FL180100036 to MJHvO). APMW and ME appreciate support by the German Research Foundation, through CRC1535 "MiBiNet". We thank Eckhard Boles for providing the EBY4000 strain.

Keren Maor-Landaw

Present address: Department of Marine Biology, The Leon H. Charney School of Marine Sciences, University of Haifa, Haifa, Israel

Marion Eisenhut

Present address: Computational Biology, Faculty of Biology, CeBiTec, Bielefeld University, Bielefeld, Germany

Giada Tortorelli

Present address: Coral Resilience Lab, Hawaiʻi Institute of Marine Biology, Kāneʻohe, HI, USA

School of Biosciences, The University of Melbourne, Parkville, VIC, Australia

Keren Maor-Landaw, Giada Tortorelli, Allison van de Meene, Madeleine J. H. van Oppen & Geoffrey I. McFadden

Institute of Plant Biochemistry, Cluster of Excellence on Plant Sciences (CEPLAS), Heinrich-Heine-University, Universitätsstraße 1, D-40225, Düsseldorf, Germany

Marion Eisenhut, Samantha Kurz & Andreas P. M. Weber

Biological Optical Microscopy Platform, The University of Melbourne, Parkville, VIC, Australia

Gabriela Segal

Australian Institute of Marine Science, Townsville, QLD, Australia

Madeleine J. H. van Oppen

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KM-L, ME, MJHvO, APMW, and GIM contributed to the conceptual development and the final edited version of the manuscript. KM-L, ME, GT, AvdM, SK, and GS conducted the experiments. KM-L, ME, MJHvO, APMW, and GIM wrote the manuscript.

Correspondence to Geoffrey I. McFadden.

The authors declare no competing interests.

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Maor-Landaw, K., Eisenhut, M., Tortorelli, G. et al. A candidate transporter allowing symbiotic dinoflagellates to feed their coral hosts. ISME COMMUN. 3, 7 (2023). https://doi.org/10.1038/s43705-023-00218-8

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Received: 02 November 2022

Revised: 10 January 2023

Accepted: 18 January 2023

Published: 28 January 2023

DOI: https://doi.org/10.1038/s43705-023-00218-8

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